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Polypyrimidine tract binding protein (PTB) associates with intronic and exonic domains to squelch nuclear export of unspliced RNA
Author(s) -
Roy Dipika,
Bhanja Chowdhury Joydip,
Ghosh Sagarmoy
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.10.005
Subject(s) - nuclear export signal , polypyrimidine tract binding protein , rna splicing , polypyrimidine tract , intron , messenger rna , rna , exon , microbiology and biotechnology , heterogeneous ribonucleoprotein particle , biology , minigene , chemistry , genetics , gene
Retention of unspliced pre‐messenger RNA (pre‐mRNA) in the nucleus is essential for cell survival. Available nuclear factors must recognize and discern between diverse export signals present in pre‐mRNA to establish an export inhibitory complex. We found that polypyrimidine domains present in both intron and exon were important for export inhibition of a minigene transcript based on hepatitis B virus pregenomic RNA. Overexpression of PTB drastically reduced export and presence of RRM4 domain seemed critical. This inhibitory network overrode stimulation from an exonic export‐facilitating element. We posit that binding of PTB to multiple loci on pre‐mRNA regulates nuclear retention.