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Delineation of the TRAK binding regions of the kinesin‐1 motor proteins
Author(s) -
Randall Thomas S.,
Moores Carolyn,
Stephenson F. Anne
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.09.049
Subject(s) - kinesin , motor protein , microbiology and biotechnology , binding site , plasma protein binding , microtubule , domain (mathematical analysis) , biology , chemistry , biochemistry , mathematics , mathematical analysis
Understanding specific cargo distribution in differentiated cells is a major challenge. Trafficking kinesin proteins (TRAKs) are kinesin adaptors. They bind the cargo binding domain of kinesin‐1 motor proteins forming a link between the motor and their cargoes. To refine the TRAK1/2 binding sites within the kinesin‐1 cargo domain, rationally designed C‐terminal truncations of KIF5A and KIF5C were generated and their co‐association with TRAK1/2 determined by quantitative co‐immunoprecipitations following co‐expression in mammalian cells. Three contributory regions forming the TRAK2 binding site within KIF5A and KIF5C cargo binding domains were delineated. Differences were found between TRAK1/2 with respect to association with KIF5A.