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Dynamic expression of miR‐126 ∗ and its effects on proliferation and contraction of hepatic stellate cells
Author(s) -
Guo Can-Jie,
Pan Qin,
Xiong Hua,
Qiao Yu-Qi,
Bian Zhao-Lian,
Zhong Wei,
Sheng Li,
Li Hai,
Shen Lei,
Hua Jing,
Ma Xiong,
Fang Jing-Yuan
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.09.047
Subject(s) - hepatic stellate cell , microbiology and biotechnology , signal transduction , microrna , extracellular matrix , biology , microcirculation , cell growth , contraction (grammar) , downregulation and upregulation , chemistry , in vitro , medicine , endocrinology , biochemistry , gene
In our previous study, miR‐126 was identified as one of the leading miRNAs that is downregulated during activation of hepatic stellate cells (HSCs). However, the roles and related mechanisms of miR‐126 in HSCs are not understood. In this study, we compared expression of miR‐126 during HSC activation both in vitro and in vivo. We also applied RNA interference to analyze the role and mechanism of miR‐126 ∗ in the activation of HSCs. Restoring HSCs with Lv‐miR‐126 ∗ resulted in decreased proliferation, accumulation of extracellular matrix components, and cell contraction, while also negatively regulating the vascular endothelial growth factor (VEGF) signal transduction pathways by partially targeted VEGF‐A. Thus, we postulate that miR‐126 may be a biological marker for the activation of HSCs, and useful for reducing intrahepatic vascular resistance and improving the sinusoidal microcirculation in chronic liver diseases.