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Distinct tRNA modifications in the thermo‐acidophilic archaeon, Thermoplasma acidophilum
Author(s) -
Tomikawa Chie,
Ohira Takayuki,
Inoue Yasushi,
Kawamura Takuya,
Yamagishi Akihiko,
Suzuki Tsutomu,
Hori Hiroyuki
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.09.021
Subject(s) - thermoplasma acidophilum , biology , chemistry , biochemistry , enzyme
Thermoplasma acidophilum is a thermo‐acidophilic archaeon. We purified tRNA Leu (UAG) from T. acidophilum using a solid‐phase DNA probe method and determined the RNA sequence after determining via nucleoside analysis and m 7 G‐specific aniline cleavage because it has been reported that T. acidophilum tRNA contains m 7 G, which is generally not found in archaeal tRNAs. RNA sequencing and liquid chromatography–mass spectrometry revealed that the m 7 G modification exists at a novel position 49. Furthermore, we found several distinct modifications, which have not previously been found in archaeal tRNA, such as 4‐thiouridine9, archaeosine13 and 5‐carbamoylmethyuridine34. The related tRNA modification enzymes and their genes are discussed.