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Role of phospholemman and the 70 kDa inhibitor protein in regulating Na + /K + ATPase activity in pulmonary artery smooth muscle cells under U46619 stimulation
Author(s) -
Dey Kuntal,
Rahaman Sayed Modinur,
Chakraborti Tapati,
Chakraborti Sajal
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.09.011
Subject(s) - chemistry , inhibitor protein , stimulation , gene isoform , atpase , microbiology and biotechnology , biochemistry , enzyme , endocrinology , biology , gene
Treatment of bovine pulmonary smooth muscle cells with U46619 inhibited the Na + /K + ATPase activity in two parallel pathways: one of which is mediated via glutathionylation of the pump and the other by augmenting the inhibitory activity of the 70 kDa inhibitor protein of Na + /K + ATPase. Although phospholemman deglutathionylates the pump leading to its activation, the inhibitor is responsible for irreversible inhibition of Na + /K + ATPase in an isoform specific manner during treatment of the cells with U46619.