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Optical manipulation of Saccharomyces cerevisiae cells reveals that green light protection against UV irradiation is favored by low Ca 2+ and requires intact UPR pathway
Author(s) -
Farcasanu Ileana C.,
Mitrica Radu,
Cristache Ligia,
Nicolau Ioana,
Ruta Lavinia L.,
Paslaru Liliana,
Comorosan Sorin
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.09.008
Subject(s) - saccharomyces cerevisiae , irradiation , chemistry , biophysics , photochemistry , radiochemistry , yeast , biochemistry , physics , biology , nuclear physics
Optical manipulation of Saccharomyces cerevisiae cells with high density green photons conferred protection against the deleterious effects of UV radiation. Combining chemical screening with UV irradiation of yeast cells, it was noted that the high density green photons relied on the presence of intact unfolded protein response (UPR) pathway to exert their protective effect and that the low Ca 2+ conditions boosted the effect. UPR chemical inducers tunicamycin, dithiotreitol and calcium chelators augmented the green light effect in a synergic action against UV‐induced damage. Photo‐manipulation of cells was a critical factor since the maximum protection was achieved only when cells were pre‐exposed to green light.