Solution NMR determination of hydrogen bonding and base pairing between the  glyQS  T box riboswitch Specifier domain and the anticodon loop of tRNA Gly | Zendy

Chang Andrew T. | Zendy; Nikonowicz Edward P. | Zendy

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Solution NMR determination of hydrogen bonding and base pairing between the glyQS T box riboswitch Specifier domain and the anticodon loop of tRNA Gly

Author(s) -

Chang Andrew T.,

Nikonowicz Edward P.

Publication year - 2013

Publication title -

febs letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.593

H-Index - 257

eISSN - 1873-3468

pISSN - 0014-5793

DOI - 10.1016/j.febslet.2013.09.003

Subject(s) - transfer rna , base pair , riboswitch , biology , stereochemistry , chemistry , rna , biochemistry , dna , gene , non coding rna

In Gram‐positive bacteria the tRNA‐dependent T box riboswitch regulates the expression of many amino acid biosynthetic and aminoacyl‐tRNA synthetase genes through a transcription attenuation mechanism. The Specifier domain of the T box riboswitch contains the Specifier sequence that is complementary to the tRNA anticodon and is flanked by a highly conserved purine nucleotide that could result in a fourth base pair involving the invariant U 33 of tRNA. We show that the interaction between the T box Specifier domain and tRNA consists of three Watson–Crick base pairs and that U 33 confers stability to the complex through intramolecular hydrogen bonding. Enhanced packing within the Specifier domain loop E motif may stabilize the complex and contribute to cognate tRNA selection.

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