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Aminoacylation of tRNA 2′‐ or 3′‐hydroxyl by phosphoseryl‐ and pyrrolysyl‐tRNA synthetases
Author(s) -
Englert Markus,
Moses Sarath,
Hohn Michael,
Ling Jiqiang,
O‧Donoghue Patrick,
Söll Dieter
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.08.037
Subject(s) - aminoacylation , transfer rna , aminoacyl trna synthetase , amino acyl trna synthetases , chemistry , biochemistry , rna , gene
Class I and II aminoacyl‐tRNA synthetases (AARSs) attach amino acids to the 2′‐ and 3′‐OH of the tRNA terminal adenosine, respectively. One exception is phenylalanyl‐tRNA synthetase (PheRS), which belongs to Class II but attaches phenylalanine to the 2′‐OH. Here we show that two Class II AARSs, O ‐phosphoseryl‐ (SepRS) and pyrrolysyl‐tRNA (PylRS) synthetases, aminoacylate the 2′‐ and 3′‐OH, respectively. Structure‐based‐phylogenetic analysis reveals that SepRS is more closely related to PheRS than PylRS, suggesting that the idiosyncratic feature of 2′‐OH acylation evolved after the split between PheRS and PylRS. Our work completes the understanding of tRNA aminoacylation positions for the 22 natural AARSs.