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Differential expression of miR‐195 in esophageal squamous cell carcinoma and miR‐195 expression inhibits tumor cell proliferation and invasion by targeting of Cdc42
Author(s) -
Fu Min-gen,
Li Shuo,
Yu Ting-ting,
Qian Li-juan,
Cao Ri-sheng,
Zhu Hong,
Xiao Bin,
Jiao Chun-hua,
Tang Na-na,
Ma Jing-jing,
Hua Jie,
Zhang Wei-feng,
Zhang Hong-jie,
Shi Rui-hua
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.08.036
Subject(s) - microrna , cdc42 , cancer research , ectopic expression , carcinogenesis , cell growth , cell cycle , cell , biology , suppressor , cell migration , cell culture , gene , genetics , biochemistry
MicroRNAs (miRNA) have played an important role in carcinogenesis. In this study, Agilent miRNA microarray was used to identify differentially expressed miRNAs in esophageal squamous cell carcinoma (ESCC) tissues and miR‐195 was downregulated in ESCC compared with normal esophageal tissues. Moreover, Cdc42 was confirmed as target gene of miR‐195. Ectopic expression of miR‐195 in ESCC cells significantly downregulated Cdc42 by directly binding its 3′ untranslated regions, and induced G1 cell cycle arrest, leading to a significant decrease in cell growth, migration, and invasion in vitro. Therefore, our findings demonstrated that miR‐195 may act as a tumor suppressor in ESCC by targeting Cdc42.