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Phylogenetic analysis and substrate specificity of GH2 β‐mannosidases from Aspergillus species
Author(s) -
Reddy Sumitha K.,
Rosengren Anna,
Klaubauf Sylvia,
Kulkarni Tejas,
Karlsson Eva Nordberg,
de Vries Ronald P.,
Stålbrand Henrik
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.08.029
Subject(s) - clade , biology , aspergillus , aspergillus nidulans , glycoside hydrolase , aspergillus niger , phylogenetic tree , phylogenetics , microbiology and biotechnology , genetics , gene , biochemistry , mutant
Phylogenetic analysis of glycoside hydrolase family 2 including Aspergillus sequences and characterised β‐mannosidases from other organisms, clusters putative Aspergillus β‐mannosidases in two distinct clades (A and B). Aspergillus species have at least one paralog in each of the two clades. It appears that clade A members are extracellular and clade B members intracellular. Substrate specificity analysis of MndA of Aspergillus niger (clade A) and MndB of Aspergillus nidulans (clade B) show that MndB, in contrast to MndA, does not hydrolyse polymeric mannan and has probably evolved to hydrolyse small unbranched β‐mannosides like mannobiose. A 3D‐model of MndB provides further insight.