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Sal‐like 4 ( SALL4 ) suppresses CDH1 expression and maintains cell dispersion in basal‐like breast cancer
Author(s) -
Itou Junji,
Matsumoto Yoshiaki,
Yoshikawa Kiyotsugu,
Toi Masakazu
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.07.049
Subject(s) - cdh1 , gene knockdown , microbiology and biotechnology , cell , cancer cell , biology , cell adhesion , cancer research , cancer , cell culture , cadherin , genetics
In cell cultures, the dispersed phenotype is indicative of the migratory ability. Here we characterized Sal‐like 4 ( SALL4 ) as a dispersion factor in basal‐like breast cancer. Our shRNA‐mediated SALL4 knockdown system and SALL4 overexpression system revealed that SALL4 suppresses the expression of adhesion gene CDH1 , and positively regulates the CDH1 suppressor ZEB1 . Cell behavior analyses showed that SALL4 suppresses intercellular adhesion and maintains cell motility after cell–cell interaction and cell division, which results in the dispersed phenotype. Our findings indicate that SALL4 functions to suppress CDH1 expression and to maintain cell dispersion in basal‐like breast cancer.