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The hepatitis B x antigen anti‐apoptotic effector URG7 is localized to the endoplasmic reticulum membrane
Author(s) -
Ostuni A.,
Lara P.,
Armentano M.F.,
Miglionico R.,
Salvia A.M.,
Mönnich M.,
Carmosino M.,
Lasorsa F.M.,
Monné M.,
Nilsson I.,
Bisaccia F.
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.07.042
Subject(s) - endoplasmic reticulum , microbiology and biotechnology , cytosol , subcellular localization , stim1 , apoptosis , glycosylation , biology , effector , n linked glycosylation , chemistry , biochemistry , glycoprotein , enzyme , cytoplasm , glycan
Hepatitis B x antigen up‐regulates the liver expression of URG7 that contributes to sustain chronic virus infection and to increase the risk for hepatocellular carcinoma by its anti‐apoptotic activity. We have investigated the subcellular localization of URG7 expressed in HepG2 cells and determined its membrane topology by glycosylation mapping in vitro . The results demonstrate that URG7 is N‐glycosylated and located to the endoplasmic reticulum membrane with an N lumen –C cytosol orientation. The results imply that the anti‐apoptotic effect of URG7 could arise from the C‐terminal cytosolic tail binding a pro‐apoptotic signaling factor and retaining it to the endoplasmic reticulum membrane.