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LMO4 modulates proliferation and differentiation of 3T3‐L1 preadipocytes
Author(s) -
Wang Ning,
Wang Xichen,
Shi Mingxin,
Shi Hongyan,
Yan Xiaohong,
Li Hui,
Wang Shouzhi,
Wang Yuxiang
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.07.033
Subject(s) - adipogenesis , 3t3 l1 , gene knockdown , 3t3 cells , microbiology and biotechnology , biology , gene expression , microarray analysis techniques , cellular differentiation , gene , genetics , transfection , mesenchymal stem cell
Previous microarray analyses revealed that LMO4 is expressed in 3T3‐L1 preadipocytes, however, its roles in adipogenesis are unknown. In the present study, using RT‐PCR sequencing and quantitative real‐time RT‐PCR, we confirmed that LMO4 gene is expressed in 3T3‐L1 preadipocytes and its expression peaks at the early stage of 3T3‐L1 preadipocyte differentiation. Further analyses showed that LMO4 knockdown decreased the proliferation of 3T3‐L1 preadipocytes, and attenuated the differentiation of 3T3‐L1 preadipocytes, as evidenced by reduced lipid accumulation and down‐regulation of PPARγ gene expression. Collectively, our findings indicate that LMO4 is a novel modulator of adipogenesis.
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