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RNA self‐processing: Formation of cyclic species and concatemers from a small engineered RNA
Author(s) -
Petkovic Sonja,
Müller Sabine
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.06.013
Subject(s) - ribozyme , rna , ligase ribozyme , phosphodiester bond , hairpin ribozyme , concatemer , intramolecular force , vs ribozyme , chemistry , cleavage (geology) , biophysics , topology (electrical circuits) , combinatorial chemistry , stereochemistry , biochemistry , biology , gene , paleontology , mathematics , combinatorics , genome , fracture (geology)
We have engineered a self‐processing RNA, derived from the hairpin ribozyme that runs through a cascade of cleavage and ligation reactions thereby changing its topology. The first two cleavage events leave the resulting RNA with a 5′‐OH group and a 2′,3′‐cyclic phosphate. Thus, upon refolding, intramolecular ligation delivers a cyclic species. In addition, we demonstrate formation of concatemers resulting from multiple intermolecular ligations. Our results demonstrate the potential of RNA for self‐supported topology changes and support the suggestion of 2′,3′‐cyclic phosphates being suitable activated building blocks for reversible phosphodiester bond formation in the RNA world.