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The anti‐inflammatory drug celecoxib inhibits t‐type Ca 2+ currents in spermatogenic cells yet it elicits the acrosome reaction in mature sperm
Author(s) -
Balderas E.,
Sánchez-Cárdenas C.,
Chávez J.C.,
de la Vega Beltrán J.L.,
Gómez-Lagunas F.,
Treviño C.L.,
Darszon A.
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.05.068
Subject(s) - acrosome reaction , mibefradil , sperm , intracellular , chemistry , microbiology and biotechnology , pharmacology , voltage dependent calcium channel , biophysics , biology , calcium , andrology , medicine , organic chemistry
Celecoxib (Cx), an anti‐inflammatory drug designed to inhibit COX2, can affect some ion channels. T‐type (Ca V 3) channels have been implicated in sperm physiology. Here we report and characterize the Cx induced inhibition of T‐type channels in mouse spermatogenic cells. Unexpectedly, Cx can also induce the acrosome reaction (AR), an intracellular Ca 2+ ([Ca 2+ ] i ) increase and a sperm depolarization. This [Ca 2+ ] i increase possibly results from the ability Cx has to alkalinize intracellular pH (pH i ), which is known to activate the sperm specific Ca 2+ channel CatSper. As the Cx induced [Ca 2+ ] i increase is sensitive to mibefradil, a CatSper blocker, this channel may mediate the Cx‐induced Ca 2+ entry leading to the AR. Our observations demonstrate that Cx can compromise fertilization.