Premium
The eukaryotic genome, its reads, and the unfinished assembly
Author(s) -
Muñoz José Fernando,
Gallo Juan Esteban,
Misas Elizabeth,
McEwen Juan Guillermo,
Clay Oliver Keatinge
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.05.048
Subject(s) - genome , computational biology , sequence assembly , biology , genetics , evolutionary biology , gene , gene expression , transcriptome
In recent years, readily affordable short read sequences provided by next‐generation sequencing (NGS) have become longer and more accurate. This has led to a jump in interest in the utility of NGS‐only approaches for exploring eukaryotic genomes. The concept of a static, ‘finished’ genome assembly, which still appears to be a faraway goal for many eukaryotes, is yielding to new paradigms. We here motivate an object‐view concept where the raw reads are the main, fixed object, and assemblies with their annotations take a role of dynamically changing and modifiable views of that object.