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HSF2BP represses BNC1 transcriptional activity by sequestering BNC1 to the cytoplasm
Author(s) -
Wu Yujian,
Liao Shangying,
Wang Xiuxia,
Wang Si,
Wang Min,
Han Chunsheng
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.04.049
Subject(s) - cytoplasm , transcriptional activity , spermatogenesis , microbiology and biotechnology , transcription factor , biology , gene , genetics , endocrinology
Basonuclin (BNC1), a zinc finger transcriptional factor, is essential for mouse spermatogenesis. However, the regulatory mechanisms of BNC1 in spermatogenesis are poorly understood. In this study, we identified HSF2BP, a testis‐specific binding protein of HSF2, as a binding partner of BNC1 by using yeast two‐hybrid screening. HSF2BP could interact with and inhibit BNC1 transcriptional activity without affecting its expression level. Moreover, coexpression of HSF2BP with BNC1 resulted in a striking redistribution of BNC1 to the cytoplasm. These data suggest that HSF2BP may play a pivotal role in regulating BNC1 transcriptional activity and subcellular localization during spermatogenesis.