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The pH sensitivity of H 2 O 2 metabolism in skeletal muscle mitochondria
Author(s) -
Banh Sheena,
Treberg Jason R.
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.04.035
Subject(s) - mitochondrion , skeletal muscle , efflux , substrate (aquarium) , metabolism , chemistry , biochemistry , mitochondrial matrix , biophysics , enzyme , biology , cytosol , endocrinology , ecology
Mitochondria have the capacity to produce and consume H 2 O 2 . We examined H 2 O 2 metabolism in isolated rat skeletal muscle mitochondria and found that the substrate‐dependent capacity to consume extramitochondrial H 2 O 2 was markedly higher than the observed rate of H 2 O 2 efflux from mitochondria under the same conditions. The substrate‐dependent capacity to consume H 2 O 2 was sensitive to the pH of the medium and we propose that pH related differences in H 2 O 2 consumption pathways may explain inconsistencies we observed between H 2 O 2 efflux rate and the reduction state of the matrix NADH pool.