Premium
The pH sensitivity of H 2 O 2 metabolism in skeletal muscle mitochondria
Author(s) -
Banh Sheena,
Treberg Jason R.
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.04.035
Subject(s) - mitochondrion , skeletal muscle , efflux , substrate (aquarium) , metabolism , chemistry , biochemistry , mitochondrial matrix , biophysics , enzyme , biology , cytosol , endocrinology , ecology
Mitochondria have the capacity to produce and consume H 2 O 2 . We examined H 2 O 2 metabolism in isolated rat skeletal muscle mitochondria and found that the substrate‐dependent capacity to consume extramitochondrial H 2 O 2 was markedly higher than the observed rate of H 2 O 2 efflux from mitochondria under the same conditions. The substrate‐dependent capacity to consume H 2 O 2 was sensitive to the pH of the medium and we propose that pH related differences in H 2 O 2 consumption pathways may explain inconsistencies we observed between H 2 O 2 efflux rate and the reduction state of the matrix NADH pool.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom