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An Arabidopsis R2R3‐MYB transcription factor, AtMYB20, negatively regulates type 2C serine/threonine protein phosphatases to enhance salt tolerance
Author(s) -
Cui Mei Hua,
Yoo Kyoung Shin,
Hyoung Sujin,
Nguyen Ha Thi Kim,
Kim Yun Young,
Kim Hae Jin,
Ok Sung Han,
Yoo Sang Dong,
Shin Jeong Sheop
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.04.028
Subject(s) - myb , abscisic acid , transcription factor , arabidopsis , phosphatase , threonine , serine , arabidopsis thaliana , phosphorylation , wild type , psychological repression , biochemistry , pyruvate dehydrogenase phosphatase , transgene , microbiology and biotechnology , biology , chemistry , mutant , enzyme , gene expression , gene , pyruvate carboxylase
We have characterized the function of a plant R2R3‐MYB transcription factor, Arabidopsis thaliana MYB20 (AtMYB20). Transgenic plants overexpressing AtMYB20 ( AtMYB20 ‐ OX ) enhanced salt stress tolerance while repression lines ( AtMYB20 ‐ SRDX ) were more vulnerable to NaCl than wild‐type plants. Following NaCl treatment, the expressions of ABI1 , ABI2 and AtPP2CA , which encode type 2C serine/threonine protein phosphatases (PP2Cs) that act as negative regulators in abscisic acid (ABA) signaling, were suppressed in AtMYB20 ‐ OX but induced in AtMYB20 ‐ SRDX . The electrophoretic mobility shift assay results revealed that AtMYB20 binds to the promoter regions containing the MYB recognition sequence (TAACTG) and an ACGT core element of ABI1 and AtPP2CA . These findings suggest that AtMYB20 down‐regulates the expression of PP2Cs , the negative regulator of ABA signaling, and enhances salt tolerance.