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Ablation of sarcolipin decreases the energy requirements for Ca 2+ transport by sarco(endo)plasmic reticulum Ca 2+ ‐ATPases in resting skeletal muscle
Author(s) -
Bombardier Eric,
Smith Ian C.,
Vigna Chris,
Fajardo Val A.,
Tupling A. Russell
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.04.019
Subject(s) - serca , skeletal muscle , chemistry , soleus muscle , endoplasmic reticulum , atpase , medicine , biophysics , endocrinology , biochemistry , biology , enzyme
The purpose of this study was to examine the effects of sarcolipin (SLN) on sarco(endo) plasmic reticulum Ca 2+ ‐ATPase (SERCA pump) energetics in vivo and resting skeletal muscle metabolic rate. Using SLN knockout ( Sln −/− ) mice we show that SLN ablation increases the transport stoichiometry of SERCA pumps (Ca 2+ uptake/Ca 2+ ‐ATPase activity) and decreases the relative contribution of SERCA pumps to resting oxygen consumption ( VO 2 ) in soleus without affecting soleus or whole body VO 2 . These data suggest that the lower energy requirements for Ca 2+ cycling in resting skeletal muscle of Sln −/− mice do not impact significantly either skeletal muscle or whole body metabolic rate.