Premium
Klotho sensitivity of the hERG channel
Author(s) -
Munoz Carlos,
Pakladok Tatsiana,
Almilaji Ahmad,
Elvira Bernat,
Seebohm Guiscard,
Voelkl Jakob,
Föller Michael,
Shumilina Ekaterina,
Lang Florian
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.04.011
Subject(s) - herg , klotho , voltage clamp , chemistry , medicine , microbiology and biotechnology , endocrinology , electrophysiology , biology , potassium channel , kidney
Klotho, a hormone and enzyme, is a powerful regulator of ageing and life span. Klotho deficiency leads to cardiac arrythmia and sudden cardiac death. We thus explored whether klotho modifies cardiac K + ‐channel hERG. Current was determined utilizing dual electrode voltage clamp and hERG protein abundance utilizing immunohistochemistry and chemiluminescence in Xenopus oocytes expressing hERG with or without klotho. Coexpression of klotho increased cell membrane hERG‐protein abundance and hERG current at any given voltage without significantly modifying the voltage required to activate the channel. The effect of klotho coexpression was mimicked by recombinant klotho protein and reversed by β‐glucuronidase‐inhibitor d ‐saccharic acid‐1,4‐lactone.