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Retracted: MicroRNA‐222 promotes tumorigenesis via targeting DKK2 and activating the Wnt/β‐catenin signaling pathway
Author(s) -
Li Qifeng,
Shen Ke,
Zhao Yang,
He Xiaoguang,
Ma Chenkai,
Wang Lin,
Wang Baocheng,
Liu Jianwen,
Ma Jie
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.04.002
Subject(s) - wnt signaling pathway , microrna , gene silencing , catenin , glioma , carcinogenesis , cancer research , rna interference , signal transduction , biology , apoptosis , microbiology and biotechnology , chemistry , gene , rna , genetics
MiR‐222 in glioma can regulate cell cycle progression and apoptosis. However, the relationship between miR‐222 and Wnt/β‐catenin signaling pathway in glioma remains unknown. Here, we found that the Dickkopf‐2 gene ( DKK2 ) was a direct target of miR‐222 by target prediction analysis and dual luciferase reporter assay. RNA interference silencing of DKK2 proved that miR‐222 overexpression led to constitutive activation of β‐catenin through inhibition of DKK2 expression in glioma cells. Furthermore, miR‐222 siRNA significantly inhibited tumorigenesis in vivo. Finally, Western blot analysis showed that miR‐222 could regulate the expression of β‐catenin and the downstream genes of Wnt/β‐catenin signaling pathway. Taken together, our findings reveal a new regulatory mechanism of miR‐222 and suggest that miR‐222 might be a potential target in glioma therapy.