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EFA6 activates Arf6 and participates in its targeting to the Flemming body during cytokinesis
Author(s) -
Ueda Tomoko,
Hanai Ayako,
Takei Tomomi,
Kubo Keiji,
Ohgi Minako,
Sakagami Hiroyuki,
Takahashi Senye,
Shin Hye-Won,
Nakayama Kazuhisa
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.03.042
Subject(s) - cytokinesis , cleavage furrow , cleavage (geology) , microbiology and biotechnology , small gtpase , septin , chemistry , biology , cell , cell division , signal transduction , biochemistry , paleontology , fracture (geology)
The small GTPase Arf6 is transiently associated with the ingressing cleavage furrow and subsequently targeted to the Flemming body during cytokinesis, suggesting its activation around the cleavage furrow. Here, we show that EFA6 (exchange factor for Arf6) localizes on the cleavage furrow through its PH domain. Time‐lapse analysis showed that both EFA6 and Arf6 are transiently localized around the ingressing cleavage furrow, but only Arf6 is subsequently targeted to the Flemming body. Expression of an EFA6 mutant suppresses Arf6 recruitment onto the Flemming body. These results suggest that EFA6 participates in activation of Arf6 around the cleavage furrow during cytokinesis.