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Expression of a vitelline membrane protein, BmVMP 23, is repressed by bmo‐miR‐1a‐3p in silkworm, Bombyx mori
Author(s) -
Chen Anli,
Xia Dingguo,
Qiu Zhiyong,
Gao Peng,
Tang Shunming,
Shen Xingjia,
Zhu Feng,
Zhao Qiaoling
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.02.030
Subject(s) - bombyx mori , luciferase , untranslated region , downregulation and upregulation , microrna , microbiology and biotechnology , three prime untranslated region , transfection , in vitro , chemistry , messenger rna , biology , gene , biochemistry
Previous studies have shown that the 3′ UTR of BmVMP 23 was destroyed by an inserted fragment, and the BmVMP 23 expression was downregulated in the lethal egg of “Ming” ( l ‐ e m ). In this study, we found a miRNA (bmo‐miR‐1a‐3p) that matches the 3′ UTR sequence of BmVMP 23 perfectly. The relationship between bmo‐miR‐1a‐3p and the BmVMP 23 expression was examined by the co‐transfection in vitro. The luciferase assay result showed that luc expression was strongly repressed. This result inferred that bmo‐miR‐1a‐3p may downregulate BmVMP 23 expression via complementary interaction with the target sites at the 3′ UTR.