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Degradome sequencing reveals an endogenous microRNA target in C. elegans
Author(s) -
Park June Hyun,
Ahn Soungyub,
Kim Soyoung,
Lee Junho,
Nam Jin-Wu,
Shin Chanseok
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.02.029
Subject(s) - caenorhabditis elegans , microrna , biology , cleavage (geology) , untranslated region , rna , rna interference , messenger rna , psychological repression , gene , three prime untranslated region , endogeny , microbiology and biotechnology , gene expression , genetics , computational biology , biochemistry , paleontology , fracture (geology)
Caenorhabditis elegans microRNAs (miRNAs) bind to partially complementary sequences in the 3′ untranslated region of target mRNAs, resulting in translational repression through mRNA destabilization. High‐throughput sequencing of RNA cleavage fragments was performed to directly detect miRNA‐directed cleavage targets in adult stage C. elegans . From this analysis, we found that miR‐249 directed the cleavage of the ZK637.6 transcript with extensive and evolutionarily conserved complementarity in nematode. In addition, expression of the ZK637.6 transcript was strongly dependent on the expression of miR‐249. These findings may lead to a better understanding of miRNA‐mediated gene regulation in nematodes.