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Effect of cargo size and shape on the transport efficiency of the bacterial Tat translocase
Author(s) -
Whitaker Neal,
Bageshwar Umesh,
Musser Siegfried M.
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.02.015
Subject(s) - translocase , biophysics , substrate (aquarium) , membrane , chemistry , nanotechnology , materials science , biology , biochemistry , gene , ecology , chromosomal translocation
The Tat machinery translocates fully‐folded and oligomeric substrates. The passage of large, bulky cargos across an ion‐tight membrane suggests the need to match pore and cargo size, and therefore that Tat transport efficiency may depend on both cargo size and shape. A series of cargos of different sizes and shapes were generated using the natural Tat substrate pre‐SufI as a base. Four (of 17) cargos transported with significant (>20% of wild‐type) efficiencies. These results indicate that cargo size and shape significantly influence Tat transportability.