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Phosphatidic acid metabolism in rat liver cell nuclei
Author(s) -
Gaveglio Virginia L.,
Pasquaré Susana J.,
Giusto Norma M.
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.01.074
Subject(s) - phosphatidic acid , diacylglycerol lipase , monoacylglycerol lipase , lysophosphatidic acid , diacylglycerol kinase , biochemistry , phospholipase d , metabolism , lipid metabolism , sphingosine , second messenger system , ceramide , lipogenesis , phospholipase , chemistry , lipase , sphingolipid , biology , enzyme , phospholipid , protein kinase c , endocannabinoid system , membrane , apoptosis , receptor
The aim of the present research was to analyze the pathways for phosphatidic acid metabolism in purified nuclei from liver. Lipid phosphate phosphatase, diacylglycerol lipase, monoacylglycerol lipase and PA‐phospholipase type A activities were detected. The presence of lysophosphatidic acid significantly reduced DAG production while sphingosine 1‐phoshate and ceramide 1‐phosphate reduced MAG formation from PA. Using different enzymatic modulators (detergents and ions) an increase in the PA metabolism by phospholipase type A was observed. Our findings evidence an active PA metabolism in purified liver nuclei which generates important lipid second messengers, and which could thus be involved in nuclear processes such as gene transcription.