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miR‐370 is stage‐specifically expressed during mouse embryonic development and regulates Dnmt3a
Author(s) -
Qi Liu,
Hongjuan He,
Ning Gu,
Zhengbin Han,
Yanjiang Xing,
Tiebo Zeng,
Zhijun Huang,
Qiong Wu
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.01.070
Subject(s) - microrna , biology , embryogenesis , morphogenesis , luciferase , microbiology and biotechnology , dna methyltransferase , embryonic stem cell , methyltransferase , western blot , embryo , dna , methylation , genetics , gene , transfection
MicroRNAs (miRNAs) are small non‐coding RNAs that participate in a large variety of biological processes. In this paper, the spatiotemporal expression pattern of miR‐370 was characterized during mouse embryonic development, and was found to be stage‐ and tissue‐specifically expressed. In addition, through luciferase reporter assays and western blot analyses, DNA methyltransferase 3A (Dnmt3a) was identified as a directly regulated target of miR‐370. Altogether, our results indicate that miR‐370 may play important roles in the morphogenesis of diverse organs, especially brain and adrenal glands, by mediating Dnmt3a expression during mouse development.