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Activation of beta 1 but not beta 3 integrin increases cell traction forces
Author(s) -
Lin Grace L.,
Cohen Daniel M.,
Desai Ravi A.,
Breckenridge Mark T.,
Gao Lin,
Humphries Martin J.,
Chen Christopher S.
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.01.068
Subject(s) - integrin , focal adhesion , cytoskeleton , microbiology and biotechnology , fibronectin , traction (geology) , cell adhesion , chemistry , adhesion , biophysics , cell , signal transduction , biology , extracellular matrix , biochemistry , paleontology , organic chemistry
Cell‐generated traction forces induce integrin activation, leading to focal adhesion growth and cell spreading. It remains unknown, however, whether integrin activation feeds back to impact the generation of cytoskeletal tension. Here, we used elastomeric micropost arrays to measure cellular traction forces in wildtype and integrin‐null cells. We report that activation of β 1 but not β 3 integrin, by either increasing density of immobilized fibronectin or treating with manganese, elicited fibroblast spreading and cytoskeletal tension. Furthermore, this force generation required Rho kinase and myosin activity. These findings suggest that integrin activation and cell traction forces comprise a bi‐directional signaling unit of cell adhesion.