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An endogene‐resembling transgene delays the onset of silencing and limits siRNA accumulation
Author(s) -
Dadami Elena,
Moser Mirko,
Zwiebel Michele,
Krczal Gabi,
Wassenegger Michael,
Dalakouras Athanasios
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.01.045
Subject(s) - transgene , gene silencing , microbiology and biotechnology , biology , rna interference , rna , gene , genetics
In plants, transgenes are generally more sensitive against RNA silencing than endogenes are. In this study, we generated a transgene that structurally mimicks an endogene. It is composed of endogenous promoter, 5′‐UTR, introns, 3′‐UTR and terminator elements. Our data revealed that, in contrast to a conventional transgene, an endogene‐resembling transgene was more stably expressed and poorly processed into small RNAs. In addition, although both constructs triggered methylation of homologous DNA sequences at similar levels, the endogene‐resembling transgene exhibited significantly delayed onset of local and systemic silencing.