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Characterization of the intramolecular G‐quadruplex promoting activity of Est1
Author(s) -
Li Qian-Jin,
Tong Xia-Jing,
Duan Yi-Min,
Zhou Jin-Qiu
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.01.024
Subject(s) - telomere , g quadruplex , dna , telomerase , dna replication , chemistry , saccharomyces cerevisiae , oligonucleotide , intramolecular force , yeast , protein subunit , microbiology and biotechnology , biology , biophysics , biochemistry , stereochemistry , gene
In the budding yeast Saccharomyces cerevisiae , telomeric DNA includes TG 1–3 /C 1–3 A double‐stranded DNA and a protruding G‐rich overhang. Our previous studies revealed that the telomerase regulatory subunit Est1 promotes telomeric single‐stranded DNA to form intermolecular G‐quadruplex in vitro, and this activity is required for telomere replication and protection in vivo. In this study, we further characterized the G‐quadruplex promoting activity of Est1. Here we report that Est1 is able to promote the single‐stranded oligonucleotide of (TGTGTGGG) 4 , which mimics the natural telomeric DNA, to form intramolecular G‐quadruplex. Therefore, it remains possible that the intramolecular G‐quadruplex promoting activity of Est1 is biologically relevant in telomere replication in vivo.