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A transthylakoid proton gradient and inhibitors induce a non‐photochemical fluorescence quenching in unicellular algae Nannochloropsis sp.
Author(s) -
Cao Shaona,
Zhang Xiaowen,
Xu Dong,
Fan Xiao,
Mou Shanli,
Wang Yitao,
Ye Naihao,
Wang Wenqi
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.12.031
Subject(s) - photoprotection , xanthophyll , chlorophyll fluorescence , quenching (fluorescence) , photosynthesis , non photochemical quenching , chemistry , antheraxanthin , violaxanthin , biophysics , algae , photoinhibition , fluorescence , electrochemical gradient , photosystem ii , photochemistry , botany , biochemistry , lutein , biology , zeaxanthin , carotenoid , physics , quantum mechanics , membrane
Non‐photochemical quenching (NPQ) of chlorophyll fluorescence is thought to be an indicator of an essential regulation and photoprotection mechanism against high‐light stress in photosynthetic organisms. In this report, special chemicals were used to perturb the kinetics of the ΔpH build‐up and the xanthophyll cycle (XC) in Nannochloropsis sp. We found that NPQ was stimulated rapidly on exposure to high light and relaxed rapidly in darkness. The ΔpH could be obligatory for NPQ and ΔpH alone was not sufficient to induce NPQ. The XC, being strictly mediated by ΔpH, was also essential for NPQ. The results demonstrate that the mechanism of NPQ in Nannochloropsis sp. resembled that of diatoms.