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Differential roles of miR‐199a‐5p in radiation‐induced autophagy in breast cancer cells
Author(s) -
Yi Heqing,
Liang Bing,
Jia Jie,
Liang Nan,
Xu Huiying,
Ju Guizhi,
Ma Shumei,
Liu Xiaodong
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.12.027
Subject(s) - autophagy , transfection , cancer cell , regulator , microbiology and biotechnology , downregulation and upregulation , master regulator , chemistry , cancer , microrna , breast cancer , cancer research , gene , biology , apoptosis , biochemistry , genetics , transcription factor
Autophagy is a self‐degrading process that is triggered by diverse stimuli including ionizing radiation. In this study we show novel phenomena in which transfection of miR‐199a‐5p mimic significantly suppresses IR‐induced autophagy in MCF7 cells, and up‐regulates basal and IR‐induced autophagy in MDA‐MB‐231 breast cancer cells. We also identify DRAM1 and Beclin1 as novel target genes for miR‐199a‐5p. Overexpression of miR‐199a‐5p inhibits DRAM1 and Beclin1 expression in MCF7 cells, while it enhances expression of these genes in MDA‐MB‐231 cells. Furthermore, we show that miR‐199a‐5p sensitizes MDA‐MB‐231 cells to irradiation. Therefore, our data identify miR‐199a‐5p as a novel and unique regulator of autophagy, which plays an important role in cancer biology and cancer therapy.