Premium
Biomolecule delivery into canola protoplasts by centrifuging cells with microbubbles
Author(s) -
He Chuan,
Gu Quanrong,
Huang Min,
Xing James,
Chen Jie
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.12.005
Subject(s) - microbubbles , biophysics , cytoplasm , cell , dextran , membrane , viability assay , protoplast , chemistry , cell membrane , materials science , chromatography , biology , biochemistry , ultrasound , physics , acoustics
We have successfully delivered FITC and FITC‐Dextran (70, 250 kDa) into canola protoplasts by centrifuging cells with different amounts of microbubbles at variable centrifuge speed. The efficiency is around 90%, while cell viability remains high. Confocal microscopy images show that both FITC and FITC‐Dextran are scattered inside the cytoplasm and the cell nucleus. Pores are observed on canola protoplast cell membranes and cell walls when centrifuged with microbubbles, while the membrane of cells centrifuged alone remain intact and smooth. We hypothesize that the collision between the microbubbles and cells or the bursting of microbubbles are the main reasons for the formation of these pores. Biomaterials can diffuse into the cells once the pathway is created.