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Sulfation of keratan sulfate proteoglycan reduces radiation‐induced apoptosis in human Burkitt's lymphoma cell lines
Author(s) -
Nakayama Fumiaki,
Umeda Sachiko,
Ichimiya Tomomi,
Kamiyama Shin,
Hazawa Masaharu,
Yasuda Takeshi,
Nishihara Shoko,
Imai Takashi
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.12.002
Subject(s) - apoptosis , sulfation , p38 mitogen activated protein kinases , chemistry , cell culture , keratan sulfate , lymphoma , microbiology and biotechnology , burkitt's lymphoma , mapk/erk pathway , kinase , biochemistry , biology , proteoglycan , immunology , extracellular matrix , genetics
This study focuses on clarifying the contribution of sulfation to radiation‐induced apoptosis in human Burkitt's lymphoma cell lines, using 3′‐phosphoadenosine 5′‐phosphosulfate transporters (PAPSTs). Overexpression of PAPST1 or PAPST2 reduced radiation‐induced apoptosis in Namalwa cells, whereas the repression of PAPST1 expression enhanced apoptosis. Inhibition of PAPST slightly decreased keratan sulfate (KS) expression, so that depletion of KS significantly increased radiation‐induced apoptosis. In addition, the repression of all three N ‐acetylglucosamine‐6‐ O ‐sulfotransferases (CHST2, CHST6, and CHST7) increased apoptosis. In contrast, PAPST1 expression promoted the phosphorylation of p38 MAPK and Akt in irradiated Namalwa cells. These findings suggest that 6‐ O ‐sulfation of GlcNAc residues in KS reduces radiation‐induced apoptosis of human Burkitt's lymphoma cells.