FixK 2 , a key regulator in  Bradyrhizobium japonicum , is a substrate for the protease ClpAP in vitro | Zendy

Bonnet Mariette | Zendy; Stegmann Monika | Zendy; Maglica Željka | Zendy; Stiegeler Emanuel | Zendy; Weber-Ban Eilika | Zendy; Hennecke Hauke | Zendy; Mesa Socorro | Zendy

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FixK 2 , a key regulator in Bradyrhizobium japonicum , is a substrate for the protease ClpAP in vitro

Author(s) -

Bonnet Mariette,

Stegmann Monika,

Maglica Željka,

Stiegeler Emanuel,

Weber-Ban Eilika,

Hennecke Hauke,

Mesa Socorro

Publication year - 2013

Publication title -

febs letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.593

H-Index - 257

eISSN - 1873-3468

pISSN - 0014-5793

DOI - 10.1016/j.febslet.2012.11.014

Subject(s) - protease , bradyrhizobium japonicum , proteases , regulator , proteolysis , biology , signal transducing adaptor protein , biochemistry , chemistry , microbiology and biotechnology , enzyme , signal transduction , rhizobiaceae , bacteria , genetics , symbiosis , gene

FixK 2 is a CRP‐like transcription factor that controls the endosymbiotic lifestyle of Bradyrhizobium japonicum . The reason for its noticeable protease sensitivity was explored here. The repertoire of Clp chaperone‐proteases in B. japonicum was examined, and specifically ClpAP 1 and ClpXP 1 were purified and tested. FixK 2 was found to be degraded by ClpAP 1 but not by ClpXP 1 . Degradation was inhibited by the ClpS 1 adaptor protein, indicating that FixK 2 is a direct substrate for ClpAP 1 . The last 12 amino acids of FixK 2 appeared to be recognized by ClpA. The results suggest that the ClpAP system is involved in the cellular turnover of FixK 2 .

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