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Sim3 shares some common roles with the histone chaperone Asf1 in fission yeast
Author(s) -
Tanae Katsuhiro,
Horiuchi Tomitaka,
Yamakawa Takuya,
Matsuo Yuzy,
Kawamukai Makoto
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.10.020
Subject(s) - microbiology and biotechnology , mutant , chaperone (clinical) , chromatin , chemistry , histone , fission , yeast , biology , dna , biochemistry , gene , physics , quantum mechanics , neutron , medicine , pathology
An H3/H4 histone chaperone, Asf1, plays an essential role in maintaining genomic stability in many species, including fission yeast. Here, we showed that overexpression of a CENP‐A chaperone Sim3 suppressed the temperature sensitive phenotype of asf1–33 and asf1–30 mutants and the defect in chromatin structure, and prevented the accumulation of DNA damage in asf1–33 mutants at high temperatures. Furthermore, asf1–33 and Δsim3 were synthetic lethal. Consistent with this, shutdown of sim3 expression in asf1–33 Δsim3 double mutants that contained extragenic sim3 resulted in growth retardation. In addition, the Δsim3 mutant displayed sensitivity to thiabendazol and hydroxyurea , which suggests that Sim3 plays a general role in maintaining chromatin structure. Our results suggest a possibility that Sim3 functions as a histone chaperone.