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MicroRNA 497 modulates interleukin 1 signalling via the MAPK/ERK pathway
Author(s) -
Zheng Dongling,
Radziszewska Anna,
Woo Patricia
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.10.014
Subject(s) - luciferase , microrna , transfection , mapk/erk pathway , reporter gene , microbiology and biotechnology , chemistry , transcription factor , hela , regulation of gene expression , gene expression , transcription (linguistics) , gene , biology , signal transduction , biochemistry , cell , linguistics , philosophy
The MAPK/ERK signalling pathway has been described to mediate IL‐1 induction of target genes and is known to be regulated by microRNAs (miRNA). We describe a novel miRNA regulating the expression of the MEK1 gene and how it impacts IL‐1 induced IL‐6 transcription. miR‐497 was predicted to target MEK1 3′UTR using bioinformatic tools. Transfection of miR‐497 into HeLa cells inhibited MEK1 protein expression by 50%. In transient transfection experiments, the luciferase activity of a MEK1 3′UTR luciferase reporter construct was reduced in the presence of miR‐497, and mutation of the predicted miR‐497 binding site restored activity. miR‐497 also decreased protein levels of RAF1 and ERK1 but not ERK2. Addition of miR‐497 was further shown to inhibit IL‐1 induced IL‐6 gene transcription.