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The 5′ untranslated region of Apaf‐1 mRNA directs translation under apoptosis conditions via a 5′ end‐dependent scanning mechanism
Author(s) -
Andreev Dmitri E.,
Dmitriev Sergey E.,
Zinovkin Roman,
Terenin Ilya M.,
Shatsky Ivan N.
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.10.010
Subject(s) - internal ribosome entry site , eif4e , eif4g , untranslated region , translation (biology) , five prime untranslated region , messenger rna , microbiology and biotechnology , three prime untranslated region , eukaryotic translation , biology , chemistry , genetics , gene
We have previously shown that translation driven by the 5′ UTR of Apaf‐1 mRNA is relatively efficient in the absence of m7G‐cap, but no IRES is involved. Nevertheless, it may be speculated that a “silent” IRES is activated under apoptosis conditions. Here, we show that translation of the mRNA with the Apaf‐1 5′ UTR is relatively resistant to apoptosis induced by etoposide when eIF4E is sequestered by 4E‐BP and eIF4G is partially cleaved. However, translation under these conditions remains governed by 5′ end‐dependent scanning. We hypothesize that the observed phenomenon is based on the intrinsic low cap‐dependence of the Apaf‐1 5′ UTR.