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Drosophila miR‐5 suppresses Hedgehog signaling by directly targeting Smoothened
Author(s) -
Wu Long-Fei,
Gao Lei,
Hou Xiao-Meng,
Zhang Qing-Hai,
Li Shen,
Yang Yong-Fei,
Lin Xin-Hua
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.10.008
Subject(s) - smoothened , hedgehog , hedgehog signaling pathway , microbiology and biotechnology , microrna , signal transduction , biology , luciferase , chemistry , genetics , gene , transfection
Hedgehog (Hh) signaling plays many important roles in developmental processes and cancers. Smoothened (Smo) is an important signal transducer in the Hh pathway, and its expression is tightly regulated by several different post‐transcriptional mechanisms. However, whether microRNAs (miRNAs) are involved in Smo regulation is still unclear. Here, we found that miR‐5 acts as a suppressor of the Hh pathway by targeting Smo. Through in vivo sensor assay and in vitro luciferase assay, we found that miR‐5 downregulates Smo through directly binding to its 3′UTR. Moreover, our data indicated Costal‐2 (Cos2) and Fused (Fu) do not play a role in the reduction of Smo mediated by miR‐5. Furthermore, we determined that miR‐5 not involved in Notch or Dpp signaling pathways by detecting target gene expression. Together, our results indicate that miR‐5 can specifically suppress Hh signaling by directly targeting Smo in Drosophila .