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Tsp66E , the Drosophila KAI1 homologue, and Tsp74F function to regulate ovarian follicle cell and wing development by stabilizing integrin localization
Author(s) -
Han Seung Yeop,
Lee Minjung,
Hong Yoon Ki,
Hwang Soojin,
Choi Gahee,
Suh Yoon Seok,
Park Seung Hwan,
Lee Soojin,
Lee Sang-Hee,
Chung Jongkyeong,
Baek Sung Hee,
Cho Kyoung Sang
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.09.044
Subject(s) - microbiology and biotechnology , cell polarity , mutant , integrin , biology , cd49c , polarity (international relations) , endocytic cycle , integrin, beta 6 , cell , gene , endocytosis , genetics
The metastasis suppressor KAI1/CD82 has been implicated in various cellular processes; however, its function in development is not fully understood. Here, we generated and characterized mutants of Tsp66E and Tsp74F , which are Drosophila homologues of KAI1/CD82 and Tspan11 , respectively. These mutants exhibited egg elongation defects along with disturbed integrin localization and actin polarity. Moreover, the defects were enhanced by mutation of inflated, an αPS2 integrin gene. Mutant ovaries had elevated αPS2 integrin levels and reduced endocytic trafficking. These results suggest that Drosophila KAI1/CD82 affects the polarized localization and the level of integrin, which may contribute to epithelial cell polarity.