Premium
Phosphorylation of measles virus phosphoprotein at S86 and/or S151 downregulates viral transcriptional activity
Author(s) -
Sugai Akihiro,
Sato Hiroki,
Yoneda Misako,
Kai Chieko
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.09.021
Subject(s) - phosphoprotein , phosphorylation , measles virus , transcription (linguistics) , nucleoprotein , viral protein , viral structural protein , biology , microbiology and biotechnology , virology , protein phosphorylation , subacute sclerosing panencephalitis , viral replication , rna polymerase ii , chemistry , protein kinase a , virus , viral entry , measles , gene expression , biochemistry , promoter , gene , linguistics , philosophy , vaccination
Measles virus phosphoprotein (P protein) is a cofactor of the viral RNA polymerase (L protein) that associates with the nucleoprotein–RNA complex to support viral transcription and replication. Here, we report a significant inverse correlation between the phosphorylation level of MV‐P protein and viral transcriptional activity. Upregulation of P protein phosphorylation resulted in reduction of viral transcription. Additionally, we found that strong phosphorylation at S86 and S151 of P protein, which may be generally prevented by association with nucleoprotein, downregulates the viral transcriptional activity. These findings suggest that P protein is involved in regulation of viral transcription through changes in its phosphorylation status.