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Regulation of mean and noise of the in vivo kinetics of transcription under the control of the lac/ara‐1 promoter
Author(s) -
Kandhavelu Meenakshisundaram,
Lloyd-Price Jason,
Gupta Abhishekh,
Muthukrishnan Anantha-Barathi,
Yli-Harja Olli,
Ribeiro Andre S.
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.09.014
Subject(s) - lac operon , kinetics , inducer , transcription (linguistics) , in vivo , limiting , biology , chemistry , microbiology and biotechnology , gene expression , biophysics , gene , biochemistry , genetics , physics , engineering , mechanical engineering , linguistics , philosophy , quantum mechanics
The kinetics of transcription initiation in Escherichia coli depend on the duration of two rate‐limiting steps, the closed and the open complex formation. In a lac promoter variant, P lac/ara‐1 , the kinetics of these steps is controlled by IPTG and arabinose. From in vivo single‐RNA measurements, we find that induction affects the mean and normalized variance of the intervals between consecutive RNA productions. Transcript production is sub‐Poissonian in all conditions tested. The kinetics of each step is independently controlled by a different inducer. We conclude that the regulatory mechanism of P lac/ara‐1 allows the stochasticity of gene expression to be environment‐dependent.