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The microtubule associated protein syntabulin is required for glucose‐stimulated and cAMP‐potentiated insulin secretion
Author(s) -
Ying Ying,
Li Lingyun,
Cao Wen,
Yan Dan,
Zeng Qing,
Kong Xiangchen,
Lu Lina,
Yan Meng,
Xu Xiaohui,
Qu Junle,
Su Qingning,
Ma Xiaosong
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.08.025
Subject(s) - insulin , medicine , secretion , endocrinology , microtubule , stimulation , chemistry , microbiology and biotechnology , biology
Syntabulin is a microtubule‐associated protein that mediates anterograde transport of vesicles to neuronal processes. Here, we found that syntabulin was expressed in mouse pancreas and insulin‐secreting β‐cells, and that it partially co‐localized with microtubule and insulin‐containing granules. The association of syntabulin with these organelles increased upon glucose stimulation. Knock‐down of syntabulin by shRNA reduced both basal and glucose‐stimulated insulin secretion, and diminished cAMP‐Epac2 and cAMP‐PKA potentiated insulin secretion. Additionally, syntabulin was preferentially phosphorylated by the Epac2 agonist 8‐pCPT‐2′‐ O ‐Me‐cAMP, suggesting that syntabulin could be a novel effector of Epac2 and play a critical role in cAMP‐enhanced insulin secretion.