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Direct reprogramming of terminally differentiated B cells into erythroid lineage
Author(s) -
Sadahira Ken,
Fukuchi Yumi,
Kunimono Hiroyoshi,
Sakurai Masatoshi,
Ikeda Yasuo,
Okamoto Shinichiro,
Nakajima Hideaki
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.08.019
Subject(s) - reprogramming , biology , lineage (genetic) , haematopoiesis , microbiology and biotechnology , transcription factor , progenitor cell , cellular differentiation , stem cell , gene , genetics
Hematopoietic progenitors have been shown to retain plasticity and switch lineages by appropriate stimuli. However, mature blood cells hardly showed such differentiation plasticity. In this paper, we tried to reprogram mature B cells into erythroid lineage by expressing various hematopoietic transcription factors. Among various factors, GATA‐1, SCL together with CCAAT/enhancer binding protein (C/EBP) α turned out to be a minimal set of factors that efficiently reprogrammed terminally differentiated mature B cells into erythroid lineage, as evidenced by colony forming assays and erythroid‐specific gene expressions. This study sets an avenue to generate autologous erythrocytes from peripheral B cells.