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A conserved regulatory element located far downstream of the gls locus modulates gls expression through chromatin loop formation during myogenesis
Author(s) -
Yuasa Katsutoshi,
Takeda Shin'ichi,
Hijikata Takao
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.07.074
Subject(s) - myogenesis , chromatin , c2c12 , biology , chromosome conformation capture , promoter , microbiology and biotechnology , gene expression , regulation of gene expression , gene , myocyte , chromatin immunoprecipitation , regulatory sequence , genetics , enhancer
Chromatin loops formed between distant regulatory elements and promoters modulate gene expression. We identified a novel distant regulatory element located approximately 120 kb downstream of the gls promoter, and examined its regulatory relevance to gls gene expression in C2C12 cells by a chromosome conformation capture assay. The distant element physically interacted with the gls promoter in myoblasts but not in myotubes. Semiquantitative analysis by real‐time PCR showed more abundant gls transcripts in myoblasts than in myotubes. These findings suggest that this distant element differentially regulates gls gene expression through dynamic formation and abrogation of a chromatin loop during myogenesis.