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Oxygen partial pressure modulates 67‐kDa laminin receptor expression, leading to altered activity of the green tea polyphenol, EGCG
Author(s) -
Tsukamoto Shuntaro,
Yamashita Shuya,
Kim Yoon Hee,
Kumazoe Motofumi,
Huang Yuhui,
Yamada Koji,
Tachibana Hirofumi
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.07.065
Subject(s) - polyphenol , cancer cell , catechin , green tea , receptor , laminin , epigallocatechin gallate , chemistry , microbiology and biotechnology , biochemistry , cancer , biology , cell , food science , antioxidant , genetics
(−)‐Epigallocatechin‐3‐ O ‐gallate (EGCG) exhibits anti‐tumor activity mediated via the 67‐kDa laminin receptor (67LR). In this study, we found that 67LR protein levels are reduced by exposure to low O 2 levels (5%), without affecting the expression of HIF‐1α. We also found that EGCG‐induced anti‐cancer activity is abrogated under low O 2 levels (5%) in various cancer cells. Notably, treatment with the proteasome inhibitor, prevented down‐regulation of 67LR and restored sensitivity to EGCG under 5% O 2 . In summary, 67LR expression is highly sensitive to O 2 partial pressure, and the activity of EGCG can be regulated in cancer cells by O 2 partial pressure.