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Coilin levels modulate cell cycle progression and γH2AX levels in etoposide treated U2OS cells
Author(s) -
Velma Venkatramreddy,
Carrero Zunamys I.,
Allen Carmen B.,
Hebert Michael D.
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.07.054
Subject(s) - cajal body , etoposide , microbiology and biotechnology , dna damage , biology , cancer research , genetics , dna , gene , rna , chemotherapy , rna splicing
Coilin is considered the Cajal body (CB) marker protein. In this report, we investigated the role of coilin in the DNA damage response and found that coilin reduction correlated with significantly increased levels of soluble γH2AX in etoposide treated U2OS cells. Additionally, coilin levels influenced the proliferation rate and cell cycle distribution of cells exposed to etoposide. Moreover, coilin overexpression inhibited nucleolar localization of endogenous coilin in etoposide treated U2OS cells. Collectively, these data provide additional evidence for coilin and CBs in the DNA damage response.