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Analysis of solvent tolerance in Pseudomonas putida DOT‐T1E based on its genome sequence and a collection of mutants
Author(s) -
Udaondo Zulema,
Duque Estrella,
Fernández Matilde,
Molina Lázaro,
de la Torre Jesús,
Bernal Patricia,
Niqui José-Luis,
Pini Cecilia,
Roca Amalia,
Matilla Miguel A.,
Antonia Molina-Henares M.,
Silva-Jiménez Hortencia,
Navarro-Avilés Gloria,
Busch Andreas,
Lacal Jesús,
Krell Tino,
Segura Ana,
Ramos Juan-Luis
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.07.031
Subject(s) - pseudomonas putida , plasmid , efflux , mutant , strain (injury) , genome , biology , gene , solvent , bacteria , whole genome sequencing , genetics , computational biology , biochemistry , anatomy
Pseudomonas putida strains are prevalent in a variety of pristine and polluted environments. The genome of the solvent‐tolerant P. putida strain DOT‐T1E which thrives in the presence of high concentrations of monoaromatic hydrocarbons, contains a circular 6.3 Mbp chromosome and a 133 kbp plasmid. Omics information has been used to identify the genes and proteins involved in solvent tolerance in this bacterium. This strain uses a multifactorial response that involves fine‐tuning of lipid fluidity, activation of a general stress‐response system, enhanced energy generation, and induction of specific efflux pumps that extrude solvents to the medium. Local and global transcriptional regulators participate in a complex network of metabolic functions, acting as the decision makers in the response to solvents.