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Constitutive expression of clathrin hub hinders elicitor‐induced clathrin‐mediated endocytosis and defense gene expression in plant cells
Author(s) -
Adam T.,
Bouhidel K.,
Der C.,
Robert F.,
Najid A.,
Simon-Plas F.,
Leborgne-Castel N.
Publication year - 2012
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2012.06.053
Subject(s) - endocytosis , clathrin , microbiology and biotechnology , elicitor , biology , receptor mediated endocytosis , gene , cell , biochemistry
Endocytosis has been recently implicated in the signaling network associated with the recognition of microbes by plants. In a previous study, we showed that the elicitor cryptogein was able to induce clathrin‐mediated endocytosis (CME) in tobacco suspension cells. Herein, we investigate further the induced CME by means of a GFP‐tagged clathrin light chain and a CME inhibitor, the hub domain of clathrin heavy chain. Hub constitutive expression does affect neither cell growth nor constitutive endocytosis but abolishes cryptogein‐induced CME. Such an inhibition has no impact on early events in the cryptogein signaling pathway but reduces the expression of defense‐associated genes.